cell biolabs VPK-156說明書

cell biolabs VPK-156說明書(shu)

QuickTiter™ MuLV Core Antigen ELISA Kit

產(chan) 品描述：

將一種抗mulv p30單克隆包衣抗體(ti) 吸附在微量滴定板上。樣品或標準品中存在的mulv核心抗原（p30）與(yu) 吸附在板上的抗體(ti) 結合；添加抗mulv p30多克隆抗體(ti) 並與(yu) 第一抗體(ti) 捕獲的抗原結合。在孵育和洗滌步驟後，添加一種HRP結合的二級抗體(ti) 並結合到抗mulv p30多克隆。在洗滌過程中去除未結合的HRP結合的二級抗體(ti) ，並將與(yu) HRP反應的底物溶液加入到井中。有色產(chan) 物的形成與(yu) 樣品中存在的mulv核心抗原的量成比例。通過添加酸終止反應，並在450 nm處測量吸光度。用重組mulv p30核心抗原製備標準曲線，測定樣品濃度。

檢測方法：Colorimetric

檢測樣本：MuLV samples

試劑盒組成：

Box 1 (shipped at room temperature)

Anti-MuLV p30 Antibody Coated Plate                  96wells

Anti-MuLV p30 Polyclonal Antibody                    20 ul

Secondary Antibody, HRP Conjugate                    20 ul

Assay Diluent 50 ml

Triton X-100 Solution                       50 ml

10X Wash Buffer                                    100 ml

Substrate Solution                                    12 ml

Stop Solution                                        12ml

Substrate Solution                                    12ml

Stop Solution                                        12ml

Box 2 (shipped on blue ice packs)

Recombinant MuLV p30 Standard                        100 ul

保存條件：Upon receipt, aliquot and store the Recombinant MuLV p30 Standard at -20ºC to avoid multiple freeze/thaw cycles.  Store all other kit components at 4ºC.

標準曲線圖：

MuLV Core Antigen ELISA Standard Curve.

Purification of Recombinant MuLV p30 Protein. Lane 1: MW STDs; Lane 2: E.Coli Whole Lysate (-IPTG); Lane 3: E.Coli Whole Lysate (+IPTG); Lane 4: Crude Lysate; Lane 5: Lysate after Ni-NTA beads; Lane 6: Bead Wash; Lane 7: Elution Fraction for Recombinant MuLV p30 Standard.