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首頁 > 技術文章 > 美國Spherotech公司SPHERO™ Rainbow Calibration Particles介紹

美國Spherotech公司SPHERO™ Rainbow Calibration Particles介紹

 更新時間:2025-10-22 點擊量:420

 一、基礎信息

貨號:RCP-30-5A  

英文名稱:SPHERO™ Rainbow Calibration Particles, 8 Peaks, 3.0-3.4 μm  

中文名稱:SPHERO™彩虹校準微球(8峰,3.0-3.4微米)  

品牌:Spherotech

規格:10⁷個(ge) /mL,5mL/瓶  

中國代理商:韦德网站官方入口

Spherotech成立於(yu) 1992年,總部位於(yu) 美國伊利諾伊州,是微球產(chan) 品製造商,專(zhuan) 注於(yu) 為(wei) 生物醫學、診斷和藥物開發領域提供均一化微球解決(jue) 方案。其產(chan) 品以高精度、高穩定性和廣泛適用性著稱,被全球科研機構、生物技術公司及醫療機構廣泛采用。

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二、產(chan) 品原理

SPHERO™彩虹校準微球通過多色熒光標記技術實現對儀(yi) 器的全麵校準。每個(ge) 微球內(nei) 部均勻嵌入多種熒光染料(如FITC、PE、PE-Cy5、APC等),形成8個(ge) 熒光強度梯度的峰。這些熒光染料的激發波長覆蓋365-650nm,可適配主流流式細胞儀(yi) 的激光係統(如488nm、561nm、633nm等)。

其核心原理包括:  

1. 多通道同步校準:  

   單一微球可同時校準流式細胞儀(yi) 的前向散射(FSC)、側(ce) 向散射(SSC)及多個(ge) 熒光通道(如FL1-FL5),通過檢測8個(ge) 熒光峰的位置和強度,驗證儀(yi) 器的靈敏度、線性度和分辨率。  

2. 熒光穩定性設計:  

   染料通過物理包埋而非表麵偶聯的方式固定在微球內(nei) 部,避免了外界環境(如pH、溫度、酶解)對熒光信號的幹擾,確保長期儲(chu) 存和重複使用時的信號一致性。  

3. 對數放大器線性驗證:  

   微球的熒光強度呈對數分布,可通過配套的Excel模板(如SPHERO™ PMT Quality Control Template)生成校準曲線,定量評估儀(yi) 器對數放大器的線性誤差,確保檢測結果的準確性。

 

三、解決(jue) 實驗中的關(guan) 鍵問題

1. 儀(yi) 器標準化與(yu) 質量控製:  

   - 流式細胞儀(yi) 的光路校準:通過FSC和SSC信號調整液流穩定性和激光對齊,確保細胞大小和顆粒度檢測的準確性。  

   - 熒光通道靈敏度驗證:檢測微球的熒光強度閾值,確認儀(yi) 器對弱信號的檢測能力,避免因PMT電壓設置不當導致的假陰性結果。  

   - 線性度評估:驗證對數放大器在寬動態範圍內(nei) 的線性響應,確保高表達和低表達樣本的信號均能準確區分。  

2. 實驗數據的可比性與(yu) 重複性:  

   - 多實驗室間數據標準化:通過統一使用SPHERO™彩虹校準微球,可消除不同儀(yi) 器和實驗條件對結果的影響,實現跨平台數據的可比性。  

   - 長期實驗質量跟蹤:定期校準可監測儀(yi) 器性能變化,及時發現光路偏移、激光衰減等問題,避免實驗結果的係統性偏差。  

3. 複雜實驗設計的優(you) 化:  

   - 熒光補償(chang) 設置:通過微球的多色熒光特性,快速建立補償(chang) 矩陣,減少因熒光光譜重疊導致的信號幹擾。  

   - 多色實驗通道匹配:根據微球的熒光發射譜,合理選擇熒光染料組合,提高實驗檢測維度。  

 

四、核心優(you) 勢

1. 多用途兼容性:  

   - 適用於(yu) 主流流式細胞儀(yi) 品牌(如BD、Beckman Coulter、Cytek等),支持從(cong) 科研級到臨(lin) 床級儀(yi) 器的校準需求。  

   - 除流式細胞儀(yi) 外,還可用於(yu) 熒光顯微鏡、共聚焦顯微鏡等設備的光路校準和熒光強度標準化。  

2. 高穩定性與(yu) 可靠性:  

   - 熒光信號在2-8℃儲(chu) 存條件下可穩定保存12個(ge) 月以上,且耐受反複凍融(至少10次),降低實驗成本。  

   - 微球粒徑均一性高(CV<2%),確保檢測結果的重複性和統計學意義(yi) 。  

3. 操作簡便與(yu) 經濟性:  

   - 即用型設計,無需複雜預處理,直接取1-2滴微球懸液稀釋後即可上機檢測。  

   - 每瓶可進行約50-100次校準(根據儀(yi) 器進樣量調整),單次實驗成本顯著低於(yu) 同類產(chan) 品。  

4. 技術支持與(yu) 資源豐(feng) 富:  

   - 提供詳細的技術文檔(如STN-8、STN-14)和操作視頻,指導用戶完成校準流程及數據分析。  

   - 配套Excel模板可自動生成校準報告,簡化數據處理步驟。  

 

五、客戶群體(ti) 與(yu) 典型應用

1. 科研領域:  

   - 免疫分析:T細胞亞(ya) 群分型、細胞因子檢測、抗體(ti) 效價(jia) 評估等實驗中,確保流式細胞儀(yi) 的靈敏度和線性度。  

   - 細胞生物學:幹細胞分化研究、細胞凋亡檢測、細胞周期分析等,保證實驗數據的準確性和可重複性。  

   - 癌症研究:循環腫瘤細胞(CTC)計數、腫瘤微環境分析等,對稀有細胞的檢測精度要求高。  

2. 臨(lin) 床診斷與(yu) IVD:  

   - 血液學檢測:白血病免疫分型、血小板功能分析等,需嚴(yan) 格遵循臨(lin) 床實驗室標準化流程。  

   - 感染性疾病診斷:HIV病毒載量監測、結核杆菌抗原檢測等,確保檢測結果的一致性和合規性。  

3. 工業(ye) 質檢與(yu) 生物技術:  

   - 生物製藥:重組蛋白表達分析、病毒載體(ti) 滴度測定等,對儀(yi) 器的穩定性和準確性要求很高。  

   - 納米材料表征:納米顆粒粒徑分布檢測、熒光標記效率評估等,利用微球的熒光特性進行定量分析。  

 

六、訂貨信息

1. 產(chan) 品規格:  

   - 貨號RCP-30-5A:8峰,3.0-3.4μm,10⁷個(ge) /mL,5mL/瓶  

   - 其他可選規格:  

     - RCP-30-5:6峰,3.0-3.4μm,5mL  

     - RCP-20-5:4峰,1.8-2.2μm,5mL  

     - 超彩虹係列(Ultra Rainbow):適用於(yu) 紫外和遠紅外通道校準  

2. 儲(chu) 存條件:  

   - 2-8℃避光保存,避免冷凍。未開封狀態下有效期為(wei) 12個(ge) 月,開封後建議6個(ge) 月內(nei) 使用完畢。  

3. 訂貨渠道:  

   - 中國代理:韦德网站官方入口

4. 注意事項:  

   - 使用前需充分混勻微球懸液,避免因沉澱導致的濃度不均。  

   - 校準過程中需使用過濾後的去離子水或鞘液,防止雜質幹擾檢測結果。  

   - 如發現微球懸液出現渾濁或沉澱,應停止使用並聯係供應商更換。  


關(guan) 鍵詞:Spherotech流式細胞儀(yi) 校準微球,RCP-30-5A,8峰校準微球,3.0-3.4μm微球,熒光校準微球,流式質控微球,多峰校準微球,Ultra Rainbow微球,流式細胞術校準,熒光補償(chang) 微球,生物醫學微球,診斷用微球,聚苯乙烯微球,流式細胞儀(yi) 比對微球,尺寸標準微球,熒光強度校準,線性校準微球,靈敏度校準微球,流式細胞儀(yi) 閾值設定,細胞分選校準,抗體(ti) 包被微球,磁性微球,乳膠微球,流式細胞術耗材,科研用微球,臨(lin) 床診斷微球,流式細胞儀(yi) 維護,微球定製,Spherotech品牌微球


更多產(chan) 品信息,請聯係中國區域代理商:韦德网站官方入口

如果您對任意產(chan) 品或其他品牌感興(xing) 趣,可通過“在線留言"功能留下您的需求

(注:本文內(nei) 容基於(yu) 品牌公開資料及行業(ye) 常規信息整理,具體(ti) 以品牌信息文檔為(wei) 準。)


🔖Spherotech熱賣產(chan) 品

貨號

品名

規格

品牌

RCP-30-5A

Rainbow Calibration Particles, 8 peaks, 1E7/mL, 3.0-3.4µm, 5mL

5mL

Spherotech

URFP-30-2

UltraRainbow Fluorescent Particles 1E7/mL 3.0-3.4 um

2ml

Spherotech

NVP-60-5

Neutravidin Polystyrene Particles 0.5% w/v 6.0-8.0 um 5 mL

5mL

Spherotech

MPFc-60-5

Gt anti-Ms IgG (Fc) Polystyrene Particles, 0.5%w/v, 6.0-8.0µm, 5mL

5ml

Spherotech

URCP-38-2K

UltraRainbow Calibration Particle Kit, 3.8 +/- 0.2µm,2E6/mL,

 1x3mL (blank) & 1E7/mL, 1x2mL (fluorescent)

1x3mL

Spherotech

SVP5-60-5

Streptavidin Polystyrene Particles, 5%w/v, 6.0-8.0µm, 5mL

5mL

Spherotech

SVP-60-5

Streptavidin Polystyrene Particles, 0.5%w/v, 6.0-8.0μm

5mL

Spherotech

C62930

Calibration Beads for MoFlo Astrioses, 1E7/mL, 3.0-3.4µm, 2 mL

2mL

Spherotech

HUP-60-5

Gt anti-Hu IgG(H&L) Polystyrene Particles, 0.5%w/v, 6.0-7.9μm, 5mL

5mL

Spherotech

RCP-30-5

Rainbow Fluorescent Particles (Intensity similar to brightest peak in RCP-30-5)

5mL

Spherotech

RPFc-60-5

Goat anti-Rabbit IgG (Fc) Coated Polystyrene Particles 0.5% w,6.0-8.0µm

5 mL

Spherotech

DIGP-20-2

Anti-Digoxigenin Coated Polystyrene Particles, 2.0- 2.4µm, 0.1%w/v, 2mL

2mL

Spherotech

ACBP-70-10

AccuCount Blank, 10^6/mL, 7.0-7.9μm, 10mL

10mL

Spherotech

NVM-20-5

NeutrAvidin Coated Magnetic Particles

5mL

Spherotech

NFPPS-52-4K

NANO FLUORESCENT SIZE STANDARD, FLOW CYTOMETRY GRADE, YELLOW, NFPPS係列

4x5mL

Spherotech

URQP-38-6K

Ultra Rainbow Quantitative Particle Kit

6x2mL

Spherotech

urfp-100-2

ULTRARAINBOW FLUORESCENT PARTICLES, URFP係列超級彩虹熒光微球

2ml

Spherotech

SVP-50-5

Streptavidin Polystyrene Particles, 0.5%w/v, 5.0-5.9µm, 5mL

5mL

Spherotech

SVP-40-5

Streptavidin Coated Polystyrene Particles 0.5% w/v 4.0-4.9 μm

5mL

Spherotech

SVP-10-5

Streptavidin Polystyrene Particles, 1%w/v, 1.0-1.4um, 5mL

5mL

Spherotech

SVP-08-10

Streptavidin Polystyrene Particles 1% w/v 0.7-0.9um

10mL

Spherotech

SVM-025-5H

Streptavidin Magnetic Particles, High Iron, 0.5% w/v, 0.2-0.39 um, 5 mL

5mL

Spherotech

ACFP-50-5

AccuCount Fluorescent Particles, 10^6/mL, 5.0-5.9µm, 5mL

5mL

Spherotech

MPFc-30-5

Gt anti-Ms IgG (Fc) Polystyrene Particles, 0.5%w/v, 3.0-3.9µm, 5mL

5mL

Spherotech

PPS-6K

Polystyrene Particle Size Standard Kit, Flow Cytometry Grade, 2.5E6/mL, 

6x5mL, 2.0-2.4µm, 3.0-3.4µm, 5.0-5.9µm, 7.0-7.9µm, 8.0-12.9µm, & 13.0- 17.9µm

6x5mL

Spherotech

CMIGP-70-3K

COMPtrol Gt anti-Ms Ig(H&L) Particles Kit, 7.0-7.9µm., 3X5mL

3X5mL

Spherotech

CMX-200-10

Carboxyl Magnetic Particles, Crosslinkled, 1%w/v, 18.0-22.9µm, 10mL

10mL

Spherotech

FP-00556-2

Fluorescent Particles, Nile Red, 1%w/v, 0.04-0.06µm, 2mL

2mL

Spherotech

CMX-300-1000

Carboxyl Magnetic Particles, Crosslinked,1% w/v, 25-37 um, 1000 mL

1000mL

Spherotech

SVP-15-5

Streptavidin Polystyrene Particles 1% w/v 1.5-1.9um 5 mL

5mL

Spherotech

CP-10-10

Carboxyl Polystyrene Particles, 5%w/v, 1.0-1.4µm, 10mL

10mL

Spherotech

DDCP-70-2

Drop Delay Calibration Particles

2mL

Spherotech

DIGP-40-2

Anti-Digoxigenin Coated Polystyrene Particles, 4.0- 4.9um, 0.1%w/v, 2mL

2mL

Spherotech

PGP-08-5

Protein G Polystyrene Particles, 1%w/v, 0.7-0.9µm, 5mL

5mL

Spherotech

PAP-60-5

Protein A Polystyrene Particles, 0.5%w/v, 6.0-8.0um, 5mL

5mL

Spherotech

PAP-40-5

Protein A Polystyrene Particles, 0.5%w/v, 4.0-4.9um, 5mL

5mL

Spherotech

PAP-08-5

Protein A Polystyrene Particles, 1%w/v, 0.7-0.9µm, 5mL

5mL

Spherotech

PGP-30-5

Protein G Polystyrene Particles, 0.5%w/v, 3.0-3.4µm, 5mL

5mL

Spherotech

PM-40-10

Polystyrene Magnetic Particles, 2.5%w/v, 4.0-4.5µm, 10mL

10mL

Spherotech

PP-08-10

polystyrene particles,5%w/v,0.7-0.9um

10ml

Spherotech

PP-100-10

Polystyrene Particles, 2.5%w/v, 8.0-12.9um, 10mL

10mL

Spherotech

PP-20-10

Polystyrene Particles, 5%w/v, 2.0-2.4µm, 10mL

10mL

Spherotech

PP-30-10

Polystyrene Particles, 5%w/v, 3.0-3.4µm, 10mL

10mL

Spherotech

PP10-10-10

Polystyrene Particles, 10%w/v, 1.0-1.4µm, 10mL

10mL

Spherotech

MP-60-5

Gt anti-Ms IgG (H&L) Polystyrene Particles, 0.5%w/v, 6.0-8.0µm, 5mL

5mL

Spherotech

ACBP-20-10

AccuCount Blank Particles

10mL

Spherotech

MMSFc-40-10

Gt anti-Ms IgG (Fc) Magnetic Particles, Smooth, 1%w/v, 4.0-5.0um, 10mL

10mL

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PPX-600-10

Polystyrene Particles, Crosslinked, Research Grade, 5%w/v, 53.0-69.0µm, 10mL

10mL

Spherotech

MM-40-10

Gt anti-Ms IgG (H&L) Magnetic Particles, 1%w/v, 4.0-4.9μm, 10mL

10mL

Spherotech

HUP-100-5

Gt anti-Hu IgG(H&L) Polystyrene Particles, 0.5%w/v, 10.0-14.0µm, 5mL

5mL

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HMX-10-10

Gt anti-Hu IgG (H&L) Magnetic Particles, Crosslinked, 0.5%w/v, 1.0-2.0um, 10mL

10mL

Spherotech

FSVM-8058-2

Streptavidin Fluorescent Magnetic Particles, Pink, 0.1%w/v, 8.0-9.9μm, 2mL

2mL

Spherotech

FSVM-2058-2

Streptavidin Fluorescent Magnetic Particles, Pink, 0.1%w/v, 2.0-2.9μm, 2mL

2mL

Spherotech

FSVM-02556-2

Streptavidin Fluorescent Magnetic Particles, Nile Red, 0.1%w/v, 0.2-0.39μm, 2mL

2mL

Spherotech

FPS-15057

Fluorescent Particle Slide, Rainbow, 13.0-17.9µm

13.0-17.9um

Spherotech

FPS-10057

Fluorescent Particle Slide, Rainbow, 10.0-14.0µm

10.0-14.0um

Spherotech

RCP-30-5A-8

Rainbow Calibration Particles, 8 peaks, 1E7/mL, 3.0-3.4µm, 5mL

5mL

Spherotech

RFP-30-5A

Rainbow Fluorescent Particles, Mid-Range, 1E7/mL, 3.0-3.4µm, 5mL

5mL

Spherotech

FP-0256-2

Fluorescent Particles, Nile Red, 1%w/v, 0.1-0.3µm, 2mL

2mL

Spherotech

RPFc-08-20

Gt anti-Rb IgG (Fc) Polystyrene Particles, 0.25%w/v, 0.7-0.9µm, 20mL

20mL

Spherotech

RTPFC-60-5

Goat anti-Rat IgG (Fc) Coated Polystyrene Particles, Cross-adsorbed, 0.5%w/v, 6.0-8.0um, 5mL

5mL

Spherotech

FH-3056-2

Fluorescent Particles, Nile red, High Intensity, 1%w/v, 2.5-4.5µm, 2mL

2mL

Spherotech

SVFM-20-5

Streptavidin Ferromagnetic Particles, 1%w/v, 2.0-2.9um, 5mL

5mL

Spherotech

SVFP-0556-5

Streptavidin Fluorescent Particles, Nile Red, 0.1%w/v, 0.4-0.6μm 5mL

5mL

Spherotech

SVFP-6056-5

Streptavidin Fluorescent Particles, Nile Red, 0.1%w/v, 5.0-7.9µm, 5mL

5mL

Spherotech

SVFP-6062-5

Streptavidin Fluorescent Particles, Purple, 0.1%w/v, 5.0-7.9µm, 5mL

5mL

Spherotech

DIGP-08-2

Anti-Digoxigenin Coated Polystyrene Particles, 0.7- 0.9um, 0.1%w/v, 2mL

2mL

Spherotech

CMX-300-1

Carboxyl Magnetic Particles, Crosslinked, 1% w/v,25-37um

2x 1mL Samples

Spherotech

URCP-100-2

UltraRainbow Calibration Particles, 6 peaks, 8.0-12.9um, 1E7/mL, 2mL

2mL

Spherotech

TM-40-10

Biotin Magnetic Particles, 1%w/v, 4.0-4.5µm, 10mL

10mL

Spherotech

TFP-0556-5

Biotin Fluorescent Particles, Nile Red, 0.1% w/v, 0.4-0.6 um, 5 mL

5mL

Spherotech

URCP-100-2-1

Ultra Rainbow Calibration Particle, Peak 1, 8.0 - 12.9 um, 1E7/mL

2mL

Spherotech

CM-025-10H

Carboxyl Magnetic Particles, High Iron, 1.0% w/v, 0.1-0.39 um, 10 mL

10mL

Spherotech

CFM-80-5

Carboxyl Ferromagnetic Particles, 1%w/v, 8.0-8.9µm, 5mL

5mL

Spherotech

CM-05-10H

Carboxyl Magnetic Particles, High Iron, 1.0%w/v, 0.4-0.69µm, 10mL

10mL

Spherotech

URCP-100-2-6

SPHERO Ultra Rainbow Calibratior Particles,Peak 6,10.4 um, 2 mL

2mL

Spherotech

URCP-100-2-8

SPHEROm Ultra Rainbow CalibrationParticles,Peak 8,10.6 um,

2mL

Spherotech

CM-200-10

Carboxyl Magnetic Particles, 1%w/v, 18.0-22.9µm, 10mL

10mL

Spherotech

CFM-20-10

Carboxyl Ferromagnetic Particles, 1%w/v, 2.0-2.9µm, 10mL

10mL

Spherotech

CM-60-10

Carboxyl Magnetic Particles, 2.5%w/v, 6.0-8.0µm, 10mL

10mL

Spherotech

URFP-02-2

Ultra Rainbow Fluorescent Particles, ~1E10/mL, 0.1-0.3µm, 2mL

2mL

Spherotech

AUP5-002-20

Citrate Gold Particles, 5.0nM, 15-20nm, 20mL

20mL

Spherotech

CMPAK-4068-6K

Carboxyl Magnetic Blue Particle Array Kit, 6 Peaks, 1E7/mL, 4.0-4.9 um, 6x1 mL

6x1mL

Spherotech

CMPAK-5068-6K

Carboxyl Magnetic Blue Particle Array Kit, 6 peaks 107/mL 4.9-5.9 μm

6x1mL

Spherotech

CMX-300-10

Carboxyl Magnetic Particles Crosslinked 1% w/v 25-37 um 10 mL

10mL

Spherotech

VM-40-10

Avidin Magnetic Particles, 1%w/v, 4.0-4.5µm, 10mL

10mL

Spherotech

CMX-40-10

Carboxyl Magnetic Particles, Crosslinked, 2.5%w/v, 3.0-6.0µm, 10mL

10mL

Spherotech

ACURFP-38-5

ACCUCOUNT ULTRARAINBOW FLUORESCENT PARTICLES

5ml

Spherotech

CP-15-10

Carboxyl Polystyrene Particles, 5%w/v, 1.5-1.9um, 10mL

10mL

Spherotech

CP-20-10

Carboxyl Polystyrene Particles, 5%w/v, 2.0-2.4um, 10mL

10mL

Spherotech

SVMS-30-10

Streptavidin Magnetic Particles, Smooth, 1%w/v, 3.0-3.9µm, 10mL

10mL

Spherotech

SVP-100-4

Streptavidin Polystyrene Particles, 0.5% w/v, 10.0-14.0 um, 4 mL

4mL

Spherotech

CP-25-10

Carboxyl Polystyrene Particles, 5%w/v, 2.5-2.9um, 10mL

10mL

Spherotech

SVMX-300-4

Streptavidin Coated Magnetic Particles, Crosslinked 0.5% w/v 25.0-37.0 µm

4mL

Spherotech

AM-05-10H

Amino Superparamagnetic Particles, High iron 1.0%w/v, 0.4-0.69um, 10mL

10mL

Spherotech

VP-10-10

Avidin Polystyrene Particles, 5.0%w/v, 1.0-1.9µm, 10mL

10mL

Spherotech

VP-30-5

Avidin Polystyrene Particles, 0.5%w/v, 3.0-3.9µm, 5mL

5mL

Spherotech

PGP-400-5

Protein G Polystyrene Particles, 1%w/v, 38-52µm, 5mL

5mL

Spherotech

VP-60-5

Avidin Polystyrene Particles, 0.5%w/v, 6.0-8.0µm, 5mL

5mL

Spherotech