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Emfret M023-2說明書(shu)
Cat.No.:XX | M023-2 |
抗原： | 整合素αIIbbeta3（GPIIb / IIIa，CD41 / CD61） |
描述： | PE綴合的JON / A抗體(ti) 選擇性結合小鼠整聯蛋白αIIbβ3的高親(qin) 和力構象，小鼠整合素αIIbβ3是許多配體(ti) 的血小板/巨核細胞特異性受體(ti) ，包括血管性血友病因子（vWf），纖維蛋白原，纖維連接蛋白和玻連蛋白。 。 |
克隆： | JON / A. |
同種型： | 大鼠IgG2b |
形成： | PE |
應用： | FC（活動表格） |
尺寸： | 1.5毫升; 300次測試 |
價(jia) 錢： | 499.00美元 |
特異性：PE結合的jon/A抗體(ti) 選擇性地結合小鼠整合素aIIb 3 1(GPIIbIIIa，CD 41/CD 61)的高親(qin) 和力構象，這是一種由135 kDa ai組成的糖蛋白複合物。 IB鏈和90 kDa b3鏈。整合素aIIbb3是纖維蛋白原、血管性血友病因子、纖維連接蛋白和維甲酸的血小板受體(ti) ，介導血小板粘附和聚集。A型 整合素aIIbb3中的陽離子依賴性構象變化，因此喬(qiao) 恩/A-PE的結合依賴於(yu) 細胞外遊離鈣。
製備和保存：用蛋白G-Sepharose層析法從(cong) 雜交瘤細胞培養(yang) 上清液中純化抗體(ti) .該抗體(ti) 與(yu) R-生理紅蛋白(PE)在宜條件下結合。 尼迪奇。將產(chan) 品保存在4°C未稀釋，避免長時間暴露在光線下。從(cong) 裝運之日起穩定一年。別凍著。
用途：本抗體(ti) 製備方法適用於(yu) 流式細胞術應用：推薦用5μl染色~106血小板或~0.5x 106細胞，體(ti) 積為(wei) 25μl泰氏-Hepes緩衝(chong) 液。 Ng 1毫米CaCl 2室溫下孵育15 min，加入400μmol/L的PBS停止反應，30 min內(nei) 對樣品進行分析。請注意孵化時間、緩衝(chong) 條件的變化。 S，或抗體(ti) 濃度可能影響瓊恩/A-PE的結合.
警告： Sodium azide是一種可逆的氧化代謝抑製劑；因此，含有這種防腐劑的抗體(ti) 製劑不得用於(yu) 細胞培養(yang) 或動物體(ti) 內(nei) 注射。 。Sodium azide可以通過洗滌染色細胞或平板結合抗體(ti) 或在無Sodium azide緩衝(chong) 液中透析可溶性抗體(ti) 來去除。
emfret授權書(shu) ：
EMFRET Analytics—專(zhuan) 注脈管係統抗體(ti)
德國EMFRET公司專(zhuan) 注心血管和血液係統研究用抗體(ti) 研發和生產(chan) ，供應的抗體(ti) 包括清除血液中血小板、流式細胞術鑒定分析小鼠血小板表麵糖蛋白，EMFRET公司的抗體(ti) 不僅(jin) 適用於(yu) 流失分析、WB檢測，還可用於(yu) 免疫組化/免疫熒光及免疫共沉澱檢測。
Cat.No. | Antigen | Clone | Isotype | Form | Applications | Size |
| ||||||
C301 | Antibodies for Mouse Plaet Depletion | polyclonal non-immune rat immunoglobulins | Rat IgG | purif. | negative control for #R300 | 0.5 mg |
| ||||||
D200 | Two-Color Analysis of Mouse Plaet Activation | Wug.E9 JON/A | Rat IgG2b/IgG1 | FITC PE | flow cytometry | 2 x 1.0 ml; 200 tests |
| ||||||
M011-0 | GPVI | JAQ1 | Rat IgG2a | purif. | IP, IHC, WB | 0.1 mg |
| ||||||
M011-1 | GPVI | JAQ1 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
| ||||||
M021-0 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.H4 | Rat IgG2b | purif. | IP, IHC, block | 0.5 mg |
| ||||||
M021-1 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.H4 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests |
| ||||||
M022-0 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.A1 | Rat IgG1 | purif. | IP, IHC | 0.5 mg |
| ||||||
M023-2 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | JON/A | Rat IgG2b | PE | FC (activ. form) | 1.5 ml; 300 tests |
| ||||||
M024-0 | Integrin alphaIIb (GPIIb, CD41) | MwReg30 | Rat IgG1 | purif. | IP, IHC, generation of Fab fragments | 6 mg |
| ||||||
M025-0 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | purif. | IP, IHC | 0.5 mg |
| ||||||
M025-1 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
| ||||||
M025-2 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | PE | FC, IHC | 1.5 ml; 300 tests |
| ||||||
M025-3 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | DyLight 649 | FC, IHC | 1.5 ml; 300 tests |
| ||||||
M030-0 | Integrin beta3 chain (GPIIIa, CD61) | Luc.A5 | Rat IgG2a | purif. | IP, IHC, WB | 0.5 mg |
| ||||||
M031-0 | Integrin beta3 chain (GPIIIa, CD61) | Luc.H11 | Rat IgG2b | purif. | IP, IHC, WB | 0.5 mg |
| ||||||
M031-1 | Integrin beta3 chain (GPIIIa, CD61) | Luc.H11 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests |
| ||||||
M040-0 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | purif. | IP, IHC | 0.5 mg |
| ||||||
M040-1 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests |
| ||||||
M040-2 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | PE | FC, IHC | 1.5 ml; 300 tests |
| ||||||
M040-3 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | DyLight 649 | FC, IHC | 1.5 ml; 300 tests |
| ||||||
M041-0 | GPIbalpha (CD42b) | Xia.H10 | Rat IgG2a | purif. | FC, IHC | 0.5 mg |
| ||||||
M041-1 | GPIbalpha (CD42b) | Xia.H10 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
| ||||||
M042-0 | GPIbalpha (CD42b) | Xia.G7 | Rat IgG1 | purif. | IP, IHC, WB | 0.5 mg |
| ||||||
M042-1 | GPIbalpha (CD42b) | Xia.G7 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests |
| ||||||
M043-0 | GPIbalpha (CD42b) | Xia.B2 | Rat IgG2a | purif. | IP, IHC, block | 0.5 mg |
| ||||||
M043-1 | GPIbalpha (CD42b) | Xia.B2 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
| ||||||
M050-0 | GPIbbeta (CD42c) | Xia.C3 | Rat IgG2a | purif. | IP, IHC, WB | 0.5 mg |
| ||||||
M050-1 | GPIbbeta (CD42c) | Xia.C3 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
| ||||||
M051-0 | GPIX (CD42a) | Xia.B4 | Rat IgG1 | purif. | IP, IHC | 0.5 mg |
| ||||||
M051-1 | GPIX (CD42a) | Xia.B4 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests |
| ||||||
M060-0 | GPV (CD42d) | Gon.C2 | Rat IgG1 | purif. | IP, IHC | 0.5 mg |
| ||||||
M060-1 | GPV (CD42d) | Gon.C2 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests |
| ||||||
M061-1 | GPV (CD42d) | Gon.G6 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
| ||||||
M070-0 | Integrin alpha2 chain (CD49b, GPIa) | Sam.G4 | Rat IgG2b | purif. | IP, IHC, block | 0.5 mg |
| ||||||
M070-1 | Integrin alpha2 chain (CD49b, GPIa) | Sam.G4 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests |
| ||||||
M071-0 | Integrin alpha2 chain (CD49b, GPIa) | Sam.C1 | Rat IgG2b | purif. | IP, IHC | 0.5 mg |
| ||||||
M071-1 | Integrin alpha2 chain (CD49b, GPIa) | Sam.C1 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests |
| ||||||
M075-0 | Integrin alpha2 chain (CD49b, GPIa) | Sam.C1, Sam.G4, Sam.H9 | Rat IgG2b/IgG1 | purif. | IP, IHC | 0.5 mg |
| ||||||
M080-0 | Integrin alpha5 chain (CD49e) | Tap.A12 | Rat IgG1 | purif. | IP, IHC | 0.5 mg |
| ||||||
M080-1 | Integrin alpha5 chain (CD49e) | Tap.A12 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests |
| ||||||
M110-0 | CD9 | Nyn.H3 | Rat IgG2a | purif. | IP, IHC, WB | 0.5 mg |
| ||||||
M110-1 | CD9 | Nyn.H3 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests |
| ||||||
M120-0 | CD31 (PECAM-1) | Pec.H3 | Rat IgG1 | purif. | IP, IHC, WB | 0.5 mg |
| ||||||
M130-0 | CD62P (P-selectin) | Wug.E9 | Rat IgG1 | purif. | IP, IHC | 0.5 mg |
| ||||||
M130-1 | CD62P (P-selectin) | Wug.E9 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests |
| ||||||
M130-2 | CD62P (P-selectin) | Wug.E9 | Rat IgG1 | PE | FC, IHC | 1.5 ml; 300 tests |
| ||||||
P140-1 | fibrinogen | polyclonal | Rabbit IgG | FITC | FC, IHC | 1.5 ml; 300 tests |
| ||||||
P150-1 | von Willebrand Factor (vWF) | polyclonal | Rabbit IgG | FITC | FC, IHC | 1.5 ml; 300 tests |
| ||||||
P180-1 | negative control | polyclonal | Rabbit IgG | FITC | FC, IHC | 1.0 ml; 200 tests |
| ||||||
P190-1 | negative control | polyclonal | Rat IgG | FITC | FC, IHC | 1.0 ml; 200 tests |
| ||||||
P190-2 | negative control | polyclonal | Rat IgG | PE | FC, IHC | 1.0 ml; 200 tests |
| ||||||
P190-3 | negative control | polyclonal | Rat IgG | DyLight 649 | FC, IHC | 1.0 ml; 200 tests |
| ||||||
R300 | Antibodies for Mouse Plaet Depletion | polyclonal anti-GPIb alpha | Rat IgG | purif. | plaet depletion | 0.5 mg |
| ||||||
X488 | Antibodies for In Vivo Mouse Plaet Labeling | anti - GPIbbeta derivative | Rat IgG | DyLight 488 | in vivo | 100 µg |
| ||||||
X649 | Antibodies for In Vivo Mouse Plaet Labeling | anti - GPIbbeta derivative | Rat IgG | DyLight 649 | in vivo | 100 µg |
貨號 | 抗原類型 | 克隆方式 | 表型 | 產(chan) 品形式 | 應用 | 規格 | ||
C301 | 清除小鼠血小板 | polyclonal non-immune rat immunoglobulins | Rat IgG | 純化 | 清除小鼠血小板 | 0.5 mg | ||
D200 | 雙色分析血小板活性 | Wug.E9 JON/A | Rat IgG2b/IgG1 | FITC、PE | 流式細胞術 | 2 x 1.0 ml; 200 tests | ||
M011-0 | GPVI | JAQ1 | Rat IgG2a | 純化 | IP, IHC, WB | 0.1 mg | ||
M011-1 | GPVI | JAQ1 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M021-0 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.H4 | Rat IgG2b | purif. | IP, IHC, block | 0.5 mg | ||
M021-1 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.H4 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M022-0 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.A1 | Rat IgG1 | purif. | IP, IHC | 0.5 mg | ||
M023-2 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | JON/A | Rat IgG2b | PE | FC (activ. form) | 1.5 ml; 300 tests | ||
M024-0 | Integrin alphaIIb (GPIIb, CD41) | MwReg30 | Rat IgG1 | purif. | IP, IHC, generation of Fab fragments | 6 mg | ||
M025-0 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | purif. | IP, IHC | 0.5 mg | ||
M025-1 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M025-2 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | PE | FC, IHC | 1.5 ml; 300 tests | ||
M025-3 | Integrin alphaIIbbeta3 (GPIIb/IIIa, CD41/CD61) | Leo.F2 | Rat IgG2a | DyLight 649 | FC, IHC | 1.5 ml; 300 tests | ||
M030-0 | Integrin beta3 chain (GPIIIa, CD61) | Luc.A5 | Rat IgG2a | purif. | IP, IHC, WB | 0.5 mg | ||
M031-0 | Integrin beta3 chain (GPIIIa, CD61) | Luc.H11 | Rat IgG2b | purif. | IP, IHC, WB | 0.5 mg | ||
M031-1 | Integrin beta3 chain (GPIIIa, CD61) | Luc.H11 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M040-0 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | purif. | IP, IHC | 0.5 mg | ||
M040-1 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M040-2 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | PE | FC, IHC | 1.5 ml; 300 tests | ||
M040-3 | GPIbalpha (CD42b) | Xia.G5 | Rat IgG2b | DyLight 649 | FC, IHC | 1.5 ml; 300 tests | ||
M041-0 | GPIbalpha (CD42b) | Xia.H10 | Rat IgG2a | purif. | FC, IHC | 0.5 mg | ||
M041-1 | GPIbalpha (CD42b) | Xia.H10 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M042-0 | GPIbalpha (CD42b) | Xia.G7 | Rat IgG1 | purif. | IP, IHC, WB | 0.5 mg | ||
M042-1 | GPIbalpha (CD42b) | Xia.G7 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M043-0 | GPIbalpha (CD42b) | Xia.B2 | Rat IgG2a | purif. | IP, IHC, block | 0.5 mg | ||
M043-1 | GPIbalpha (CD42b) | Xia.B2 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M050-0 | GPIbbeta (CD42c) | Xia.C3 | Rat IgG2a | purif. | IP, IHC, WB | 0.5 mg | ||
M050-1 | GPIbbeta (CD42c) | Xia.C3 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M051-0 | GPIX (CD42a) | Xia.B4 | Rat IgG1 | purif. | IP, IHC | 0.5 mg | ||
| ||||||||
M051-1 | GPIX (CD42a) | Xia.B4 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M060-1 | GPV (CD42d) | Gon.C2 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M061-1 | GPV (CD42d) | Gon.G6 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M070-0 | Integrin alpha2 chain (CD49b, GPIa) | Sam.G4 | Rat IgG2b | purif. | IP, IHC, block | 0.5 mg | ||
M070-1 | Integrin alpha2 chain (CD49b, GPIa) | Sam.G4 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M071-0 | Integrin alpha2 chain (CD49b, GPIa) | Sam.C1 | Rat IgG2b | purif. | IP, IHC | 0.5 mg | ||
M071-1 | Integrin alpha2 chain (CD49b, GPIa) | Sam.C1 | Rat IgG2b | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M075-0 | Integrin alpha2 chain (CD49b, GPIa) | Sam.C1, Sam.G4, Sam.H9 | Rat IgG2b/IgG1 | purif. | IP, IHC | 0.5 mg | ||
M080-0 | Integrin alpha5 chain (CD49e) | Tap.A12 | Rat IgG1 | purif. | IP, IHC | 0.5 mg | ||
M080-1 | Integrin alpha5 chain (CD49e) | Tap.A12 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M110-0 | CD9 | Nyn.H3 | Rat IgG2a | purif. | IP, IHC, WB | 0.5 mg | ||
M110-1 | CD9 | Nyn.H3 | Rat IgG2a | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M120-0 | CD31 (PECAM-1) | Pec.H3 | Rat IgG1 | purif. | IP, IHC, WB | 0.5 mg | ||
M130-0 | CD62P (P-selectin) | Wug.E9 | Rat IgG1 | purif. | IP, IHC | 0.5 mg | ||
M130-1 | CD62P (P-selectin) | Wug.E9 | Rat IgG1 | FITC | FC, IHC | 1.5 ml; 300 tests | ||
M130-2 | CD62P (P-selectin) | Wug.E9 | Rat IgG1 | PE | FC, IHC | 1.5 ml; 300 tests | ||
P140-1 | fibrinogen | polyclonal | Rabbit IgG | FITC | FC, IHC | 1.5 ml; 300 tests | ||
P150-1 | von Willebrand Factor (vWF) | polyclonal | Rabbit IgG | FITC | FC, IHC | 1.5 ml; 300 tests | ||
P180-1 | negative control | polyclonal | Rabbit IgG | FITC | FC, IHC | 1.0 ml; 200 tests | ||
P190-1 | negative control | polyclonal | Rat IgG | FITC | FC, IHC | 1.0 ml; 200 tests | ||
P190-2 | negative control | polyclonal | Rat IgG | PE | FC, IHC | 1.0 ml; 200 tests | ||
| ||||||||
P190-3 | negative control | polyclonal | Rat IgG | DyLight 649 | FC, IHC | 1.0 ml; 200 tests | ||
R300 | Antibodies for Mouse Plaet Depletion | polyclonal anti-GPIb alpha | Rat IgG | purif. | plaet depletion | 0.5 mg | ||
X488 | Antibodies for In Vivo Mouse Plaet Labeling | anti - GPIbbeta derivative | Rat IgG | DyLight 488 | in vivo | 100 µg | ||
X649 | Antibodies for In Vivo Mouse Plaet Labeling | anti - GPIbbeta derivative | Rat IgG | DyLight 649 | in vivo | 100 µg | ||
我們(men) 公司優(you) 勢是強大的采購，
1：基本什麽(me) 都能進口，血清，抗體(ti) ，耗材，還有部分限製進口的，
2：貨品全，現經營過700多個(ge) 品牌，基本所有生物試劑耗材都可以進口，特別是冷偏的產(chan) 品那就更有優(you) 勢，
3：提供加急服務，一般1-2周到貨，超過時限加急費全免
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5：良好的信譽，大部分客戶我們(men) 提供貨到付款服務，客戶包括清華，北大 交大 複旦，中山等100多所大學，ROCHE，阿斯利康，國藥 ，fisher等500多家公司
6：我們(men) *的品牌有：Antibody Research Corporation，arcticzymes，Biorelevant，AmberGen, Inc. ，clemente-associates，clodronaiposomes，Columbia Biosesciences，enzyme research，Gene Bridges GmbH，Genovis，AmberGen, Inc. Biotechnology GmbH，Haematologic Technologies HTI Haemtech，hookelabs，Immudex，Innovative Research of America，inspiralis ，List Biological Laboratories, Inc.，lumafluor，Microsurfaces，multiplicom，nanotools，Pel-Freez Biologicals，pentapharm，progen，Protein Ark，chronolog,Inc，chronolog,IncQuickZyme Biosesciences，Teknova，TriLink BioTechnologies, Inc.，Zyagen Laboratories 等
7：我們(men) 還是invitrogen,qiagen,Midland BioProducts Corporationam,sigma;neb,roche,merck, rnd,BD, GE,pierce,BioLegend等知名*批發，歡迎合作。
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